Free-boundary electrophoresis of histones

Free Histones and the Cell Cycle

Free flow electrophoresis of chloroplasts.

Highly purified intact chloroplasts were isolated from spinach (Spinacia oleracea L.) leaves by free flow electrophoresis. Morphological and biochemical studies showed that the fraction enriched in intact chloroplasts has a higher protein to chlorophyll ratio and a higher linolenic acid content than the broken organelles of the other fraction. The intact chloroplasts prepared by electrophoresis...

Multiple determinations with moving-boundary electrophoresis.

OVING-BOUNDARY BLECTROPUORESIS is a widely used technic for the investigation of colloidal systems. A limitation of this method, however, is the considerable amount of time required for a single experiment. It is particularly time consuming where the accumulation of large amounts of data is necessary. Furthermore, in the majority of instruments only 1 experiment at a time, and usually a total o...

Zone and boundary diffusion in electrophoresis.

In chromatography, electrophoresis and sedimentation, an irreversible zone or boundary spreading is often observed which is greater than that attributable to ordinary molecular diffusion and to dispersion caused by flow in porous media. The additional spreading can often be identified with kinetic processes such as adsorption, partition, and chemical interconversion. The existence of this kinet...

Extracting histones for the specific purpose of label‐free MS

Extracting histones from cells is the first step in studies that aim to characterize histones and their post-translational modifications (hPTMs) with MS. In the last decade, label-free quantification is more frequently being used for MS-based histone characterization. However, many histone extraction protocols were not specifically designed for label-free MS. While label-free quantification has...